Association of gene polymorphism and cytokine content in the blood serum in children with allergic bronchial asthma

The study of genes controlling cytokine activities is among important tasks when assessing predisposition and revealing pathogenetic links initiation course clinical disorders. Aberrant production cytokines dysregulation immune response may be considered genetic predictors associated with differentiation functioning T helpers, being decisive importance in pathogenesis pediatric allergic bronchial asthma. Our objective was to evaluation associations between polymorphic genotypes serum levels various helper profiles the children asthma.
 We have observed 175 aged 3 11 years. Of them, we examined 75 patients diagnosed asthma (ABA) as well 100 healthy matched for age gender. All underwent general allergological examination. contents attributed Th1, Th2 Th17 were determined blood by means ELISA technique. DNA samples isolated from peripheral venous used molecular analysis. Using allele-specific PCR technique, following mutation points investigated: IFN (T-874 A), IL-4 (C-589 T), IL-6 (C-174 G), IL-17A (G- 197 TNF (G-308 A). analysis distribution occurrence gene polymorphisms carried out, odds ratio disease risk calculated. Statistical data processing out using program Statistica 10 methods descriptive, parametric non-parametric statistics, comparison unrelated groups performed qualitative characteristics HardyWeinberg equilibrium, Chi-square test ( 2).
 These studies revealed differences patterns aberrant typical Th A comparative mutant allele frequencies profiles, along determination a predominance homozygous 874A, 589T, 174G, 197A, 308A genotypes. Studies polymorphisms, features content specific helpers 1, 2, 17 alleles production, variable developing pathology development distinct phenotype.